Cloning and chromosomal localization of a gene (GPR18) encoding a novel seven transmembrane receptor highly expressed in spleen and testis

Genomics. 1997 Jun 15;42(3):462-6. doi: 10.1006/geno.1997.4752.


Using the techniques of relaxed stringency polymerase chain reaction and genomic library screening, we have isolated homologous canine and human genes that encode a novel putative seven transmembrane G-protein-linked receptor. The gene encodes an open reading frame (ORF) of 993 bp. The sequences of the canine and human ORFs are highly conserved, sharing 89% nucleotide identity and 92% amino acid similarity between the two species. Northern blot analysis demonstrates that mRNA transcripts of the gene are abundantly expressed in testis and spleen with a lesser degree of expression observed in several other tissues associated with endocrine and immunologic/hematologic function. The gene, designated GPR18, was localized to human chromosome 13q32 using fluorescence in situ hybridization.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Chromosome Mapping
  • Chromosomes, Human, Pair 13*
  • Cloning, Molecular
  • DNA, Complementary
  • Dogs
  • GTP-Binding Proteins / metabolism
  • Gene Expression
  • Humans
  • Male
  • Mice
  • Molecular Sequence Data
  • Pseudogenes
  • Receptors, Cell Surface / chemistry
  • Receptors, Cell Surface / genetics*
  • Receptors, G-Protein-Coupled*
  • Sequence Homology, Amino Acid
  • Spleen / metabolism
  • Testis / metabolism
  • Tumor Cells, Cultured


  • DNA, Complementary
  • GPR18 protein, human
  • Receptors, Cell Surface
  • Receptors, G-Protein-Coupled
  • GTP-Binding Proteins

Associated data

  • GENBANK/L42324
  • GENBANK/L42326