Abstract
The reeler Albany2 mutation (Reln(rl-Alb2) in the mouse is an allele of reeler isolated during a chlorambucil mutagenesis screen. Homozygous animals had drastically reduced concentrations of reelin mRNA, in which an 85-nt exon was absent. At the genomic level, the mutation was shown to be due to an intracisternal A-particle insertion leading to exon skipping. This appears to be the first observation of retrotransposon insertion during chlorambucil mutagenesis.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alleles
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Animals
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Base Sequence
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Cell Adhesion Molecules, Neuronal / genetics*
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Chlorambucil / pharmacology
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Exons*
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Extracellular Matrix Proteins / genetics*
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Female
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Genes, Intracisternal A-Particle*
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Homozygote
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Male
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Mice
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Mice, Inbred C3H
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Molecular Sequence Data
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Mutagenesis, Insertional
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Nerve Tissue Proteins
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RNA, Messenger
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Reelin Protein
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Serine Endopeptidases
Substances
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Cell Adhesion Molecules, Neuronal
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Extracellular Matrix Proteins
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Nerve Tissue Proteins
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RNA, Messenger
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Reelin Protein
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Chlorambucil
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Reln protein, mouse
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Serine Endopeptidases
Associated data
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GENBANK/U79726
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GENBANK/U79727