Reln(rl-Alb2), an allele of reeler isolated from a chlorambucil screen, is due to an IAP insertion with exon skipping

Genomics. 1997 Jun 15;42(3):479-82. doi: 10.1006/geno.1997.4772.

Abstract

The reeler Albany2 mutation (Reln(rl-Alb2) in the mouse is an allele of reeler isolated during a chlorambucil mutagenesis screen. Homozygous animals had drastically reduced concentrations of reelin mRNA, in which an 85-nt exon was absent. At the genomic level, the mutation was shown to be due to an intracisternal A-particle insertion leading to exon skipping. This appears to be the first observation of retrotransposon insertion during chlorambucil mutagenesis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Animals
  • Base Sequence
  • Cell Adhesion Molecules, Neuronal / genetics*
  • Chlorambucil / pharmacology
  • Exons*
  • Extracellular Matrix Proteins / genetics*
  • Female
  • Genes, Intracisternal A-Particle*
  • Homozygote
  • Male
  • Mice
  • Mice, Inbred C3H
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Nerve Tissue Proteins
  • RNA, Messenger
  • Serine Endopeptidases

Substances

  • Cell Adhesion Molecules, Neuronal
  • Extracellular Matrix Proteins
  • Nerve Tissue Proteins
  • RNA, Messenger
  • Chlorambucil
  • Serine Endopeptidases
  • reelin protein

Associated data

  • GENBANK/U79726
  • GENBANK/U79727