Isolation and chromosomal localization of GPR31, a human gene encoding a putative G protein-coupled receptor

Genomics. 1997 Jun 15;42(3):519-23. doi: 10.1006/geno.1997.4754.


The screening of a human genomic library with a chemokine receptor-like probe allowed us to obtain a putative member of the G protein-coupled receptor gene (GPCR) family, designated GPR31. Its deduced amino acid sequence encodes a polypeptide of 319 amino acids that shares 25-33% homology with members of the chemokine, purino, and somatostatin receptor gene families. Amino acid sequence comparison reveals that the best match in the protein databases is with the human orphan GPCR called HM74 (33% identity). Southern genomic analysis of the GPR31 gene shows a hybridization pattern consistent with that of a single-copy gene. Using fluorescence in situ hybridization, we have determined the chromosomal and regional localization of the GPR31 gene at 6q27. The GPR31 mRNA is expressed at low levels by several human cell lines of different cellular origins. The phylogenetic analysis suggests that the GPR31 receptor may represent a member of a new GPCR subfamily.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Southern
  • Chromosome Mapping
  • Chromosomes, Human, Pair 6*
  • DNA, Complementary
  • GTP-Binding Proteins / metabolism*
  • Genomic Library
  • Humans
  • Molecular Sequence Data
  • Receptors, Cell Surface / chemistry
  • Receptors, Cell Surface / genetics*
  • Receptors, Cytokine / genetics*
  • Receptors, Cytokine / metabolism
  • Receptors, G-Protein-Coupled*


  • DNA, Complementary
  • GPR31 protein, human
  • Receptors, Cell Surface
  • Receptors, Cytokine
  • Receptors, G-Protein-Coupled
  • GTP-Binding Proteins

Associated data

  • GENBANK/U65402
  • SWISSPROT/P32248