Sequence analysis of the 36-kb region between gntZ and trnY genes of Bacillus subtilis genome

DNA Res. 1997 Apr 28;4(2):155-9. doi: 10.1093/dnares/4.2.155.


Within the framework of an international Bacillus subtilis genome sequencing project, we have determined a 36-kb sequence covering the region between the gntZ and trnY genes. In addition to five genes sequenced and characterized previously, 27 putative protein coding sequences (open reading frame; ORF) were identified. A homology search for the newly identified ORFs revealed that six of them had similarities to known proteins. It is notable that new ORFs belonging to response-regulator aspartate phosphatase (Rap) and its regulator (Phr) families, and response regulator and sensory kinase families of two-component signal transduction systems have been identified. Furthermore, we found that some 180-bp non-coding sequence, that might be an remnant of an ancient IS element, is preserved in at least five loci of the B. subtilis genome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus subtilis / genetics*
  • Base Sequence
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA Transposable Elements
  • DNA, Bacterial* / analysis
  • Genome, Bacterial*
  • Molecular Sequence Data
  • Open Reading Frames
  • Sequence Analysis, DNA*


  • DNA Transposable Elements
  • DNA, Bacterial

Associated data

  • GENBANK/AB001488
  • GENBANK/D78193
  • GENBANK/U11882
  • GENBANK/X70356
  • GENBANK/X73124