Extracellular matrix is reduced by inhibition of transforming growth factor beta1 in pancreatitis in the rat

Gastroenterology. 1997 Jul;113(1):295-303. doi: 10.1016/s0016-5085(97)70107-0.


Background & aims: Regeneration from cerulein-induced pancreatitis is accompanied by a transient synthesis and deposition of extracellular matrix components in the rat pancreas. The pleiotropic transforming growth factor (TGF)-beta1 has been suggested to regulate extracellular matrix remodeling during regeneration from acute pancreatitis. The present study was designed to verify this hypothesis by investigating the effect of TGF-beta1 inhibition.

Methods: Experimental acute pancreatitis was induced in rats by supramaximal doses of cerulein. The biological activity of TGF-beta1 was inhibited by injections of neutralizing TGF-beta1 antibody. Changes in the content of extracellular matrix proteins, TGFs, and their messenger RNA concentrations were monitored.

Results: TGF-beta1 expression in pancreatic cells was suppressed after induction of acute pancreatitis by the application of neutralizing TGF-beta1 antibody. Immunochemical analysis showed a clear reduction of extracellular matrix formation during the regeneration of the pancreas in antibody-treated animals. The hydroxyproline content and the concentration of collagen types I and III, fibronectin on protein, and messenger RNA level were significantly reduced in the pancreas of treated animals.

Conclusions: These results provide evidence that TGF-beta1 is involved in the regulation of extracellular matrix remodeling in the rat pancreas during regeneration from acute pancreatitis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Animals
  • Blotting, Western
  • Ceruletide
  • Collagen / metabolism
  • Extracellular Matrix / physiology*
  • Extracellular Matrix Proteins / metabolism
  • Male
  • Pancreas / metabolism
  • Pancreas / physiology*
  • Pancreatitis / chemically induced
  • Pancreatitis / physiopathology*
  • RNA, Messenger / genetics
  • Rats
  • Rats, Wistar
  • Regeneration / physiology*
  • Transforming Growth Factor beta / antagonists & inhibitors
  • Transforming Growth Factor beta / immunology
  • Transforming Growth Factor beta / physiology*


  • Extracellular Matrix Proteins
  • RNA, Messenger
  • Transforming Growth Factor beta
  • Ceruletide
  • Collagen