Comparative study of Mycobacterium paratuberculosis strains isolated from Crohn's disease and Johne's disease using restriction fragment length polymorphism and arbitrarily primed polymerase chain reaction

Epidemiol Infect. 1997 Jun;118(3):227-33. doi: 10.1017/s0950268897007486.

Abstract

To obtain insights into the pathogenic mechanisms involving Mycobacterium paratuberculosis in Crohn's disease (CD) we questioned if the strains of M. paratuberculosis isolated from CD are distinguishable from those involved in Johne's disease (JD), a chronic granulomatous enteritis in cattle. Accordingly we compared human and animal strains at the DNA level, both by the analysis of restriction fragment length polymorphism (RFLP) in and around the insertion sequence IS 900 and by the arbitrarily primed chain reaction (AP-PCR). Results are in favour of a common clonal origin for the 4 strains isolated from CD and for 8 of the 11 strains isolated from cattle and sheep JD.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Blotting, Southern
  • Cattle
  • Crohn Disease / microbiology*
  • DNA Primers
  • DNA, Bacterial / genetics*
  • Humans
  • Mycobacterium avium subsp. paratuberculosis / genetics
  • Mycobacterium avium subsp. paratuberculosis / isolation & purification*
  • Mycobacterium avium subsp. paratuberculosis / pathogenicity*
  • Paratuberculosis / microbiology*
  • Polymerase Chain Reaction* / methods
  • Polymorphism, Restriction Fragment Length*

Substances

  • DNA Primers
  • DNA, Bacterial