The ribosomal protein P0 of soybean (Glycine max L. Merr.) has antigenic cross-reactivity to soybean seed lectin

Plant Mol Biol. 1997 May;34(2):295-306. doi: 10.1023/a:1005817114562.

Abstract

Soybean (Glycine max L. Merr.) mutants lacking the ability to produce the lectin normally found in soybean seeds (SBL) are designated Le-. A protein of higher molecular weight that cross-reacts with antibodies raised to SBL was found at nearly equivalent levels in roots, hypocotyls, and leaves, and at lower levels in cotyledons and dry seeds of both Le+ and Le- soybean cultivars. Earlier work suggested that this protein was a novel lectin. Clones isolated from a Le- soybean root cDNA library produced a cross-reacting protein of the same size in Escherichia coli. Sequence analysis of these clones revealed a high degree of similarity to the ribosomal protein P0. The cross-reacting protein co-purified with ribosomes, and a monoclonal antibody raised to purified brine shrimp P0 cross-reacted to the same protein. The protein showed no lectin activity in a hemagglutination assay, nor did it bind to an N-acetyl-D-galactosamine affinity column. On the basis of this evidence, we conclude that the SBL-cross-reacting protein is not a lectin but a homologue of the ribosomal protein P0. Consequently, Le- soybeans must produce a lectin that is dissimilar to SBL at both the DNA and amino acid levels and we suggest that it is this lectin which is involved in nodulation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Cross Reactions
  • DNA, Complementary / isolation & purification
  • Gene Expression Regulation, Plant
  • Lectins / chemistry
  • Lectins / genetics
  • Lectins / immunology*
  • Molecular Sequence Data
  • Plant Lectins
  • Ribosomal Proteins / biosynthesis
  • Ribosomal Proteins / genetics
  • Ribosomal Proteins / immunology*
  • Ribosomes / chemistry
  • Seeds / immunology
  • Soybean Proteins*
  • Soybeans / chemistry
  • Soybeans / genetics
  • Soybeans / immunology*
  • Tissue Distribution

Substances

  • DNA, Complementary
  • Lectins
  • Plant Lectins
  • Ribosomal Proteins
  • Soybean Proteins
  • soybean lectin

Associated data

  • GENBANK/D21130
  • GENBANK/L46848
  • GENBANK/X05466
  • GENBANK/X15206
  • GENBANK/X78213
  • GENBANK/Z33760