GM-CSF-mobilized peripheral blood CD34+ cells differ from steady-state bone marrow CD34+ cells in adhesion molecule expression

Bone Marrow Transplant. 1997 Jun;19(12):1175-81. doi: 10.1038/sj.bmt.1700814.

Abstract

To determine the effect of growth factor mobilization on the expression of adhesion molecules, we compared CD34+ progenitor cell (PC) populations from steady-state bone marrow (BM) with granulocyte-macrophage colony-stimulating factor (GM-CSF)-mobilized apheresis products (peripheral blood stem cell (PSC)) using flow cytometry. To increase the accuracy of this analysis, CD34+ cells were enriched (MiniMACS) before cytometric analysis. A significantly lower expression of very late antigen-4 (VLA-4), leukocyte function antigen-1 (LFA-1) and LFA-3 were observed on PSC compared to BM CD34+ cells. In addition, significantly lower mean fluorescence intensity (MFI) of VLA-4, VLA-5, intercellular adhesion molecule-1 (ICAM-1), and sialyl Lewisx were observed on PSC as compared to BM CD34+ cells. Significantly higher levels of L-selectin and CD44 expression were observed on PSC as compared to BM CD34+ cells based on frequency and MFI (P < or = 0.05). In addition, the duration of GM-CSF administration or number of prior aphereses had no effect on adhesion molecule expression. These data suggest that decreased expression of adhesion molecules including VLA-4, LFA-1, ICAM-1 and LFA-3 play a role in PC mobilization. Based on these studies, we suggest that PC mobilization occurs as a stochastic process and is associated with the selection of CD34+ cells with low adhesion molecule expression.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Aged
  • Antigens, CD34 / metabolism*
  • Bone Marrow / immunology*
  • Bone Marrow Cells
  • CD58 Antigens / metabolism
  • Cell Adhesion Molecules / metabolism*
  • Female
  • Flow Cytometry
  • Granulocyte-Macrophage Colony-Stimulating Factor / administration & dosage*
  • Hematopoietic Stem Cell Transplantation
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / drug effects*
  • Hematopoietic Stem Cells / immunology*
  • Hodgkin Disease / therapy
  • Humans
  • Integrin alpha4beta1
  • Integrins / metabolism
  • Intercellular Adhesion Molecule-1 / metabolism
  • Leukapheresis
  • Lymphocyte Function-Associated Antigen-1 / metabolism
  • Lymphoma, Non-Hodgkin / therapy
  • Male
  • Middle Aged
  • Oligosaccharides / metabolism
  • Receptors, Lymphocyte Homing / metabolism
  • Sialyl Lewis X Antigen
  • Transplantation, Autologous

Substances

  • Antigens, CD34
  • CD58 Antigens
  • Cell Adhesion Molecules
  • Integrin alpha4beta1
  • Integrins
  • Lymphocyte Function-Associated Antigen-1
  • Oligosaccharides
  • Receptors, Lymphocyte Homing
  • Sialyl Lewis X Antigen
  • Intercellular Adhesion Molecule-1
  • Granulocyte-Macrophage Colony-Stimulating Factor