Failure of Lymphopoiesis After Adoptive Transfer of NF-kappaB-deficient Fetal Liver Cells

Immunity. 1997 Jun;6(6):765-72. doi: 10.1016/s1074-7613(00)80451-3.

Abstract

Mice deficient in the p65 subunit of NF-kappaB die during fetal development. Introduction of p50/p65-deficient fetal liver cells into lethally irradiated hosts resulted in a severe deficit of fetal liver-derived lymphocytes and their immediate precursors but an overabundance of fetal liver-derived granulocytes. Surprisingly, simultaneous transplantation of wild-type bone marrow cells rescued the production of p50/p65-deficient lymphocytes. Expression of immunoglobulin K light chains on these rescued NF-kappaB-deficient B lymphocytes was normal. These results suggest that while p50 and p65 do not regulate the maturation of pre-B cells, NF-kappaB mediates the development or survival of an early lymphocyte precursor through regulation of an extracellular factor.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adoptive Transfer
  • Animals
  • B-Lymphocytes / cytology*
  • Bone Marrow Cells
  • Cells, Cultured
  • Flow Cytometry
  • Gene Rearrangement, B-Lymphocyte
  • Granulocytes / cytology*
  • Hematopoiesis*
  • Hematopoietic Stem Cells / cytology*
  • Immunoglobulin kappa-Chains / genetics
  • Immunoglobulin lambda-Chains / genetics
  • Liver / embryology
  • Mice
  • Mice, Inbred C57BL
  • NF-kappa B / deficiency*
  • NF-kappa B p50 Subunit
  • Radiation Chimera
  • Spleen / cytology
  • T-Lymphocytes / cytology*
  • Thymus Gland / cytology
  • Transcription Factor RelA

Substances

  • Immunoglobulin kappa-Chains
  • Immunoglobulin lambda-Chains
  • NF-kappa B
  • NF-kappa B p50 Subunit
  • Transcription Factor RelA