Characterization of thiI, a new gene involved in thiazole biosynthesis in Salmonella typhimurium

J Bacteriol. 1997 Jul;179(13):4399-402. doi: 10.1128/jb.179.13.4399-4402.1997.


Thiamine pyrophosphate (TPP) is a required cofactor in Salmonella typhimurium that is generated de novo by the condensation of 4-amino-5-hydroxymethyl pyrimidine (HMP) pyrophosphate and 4-methyl-5-(beta-hydroxyethyl)-thiazole (THZ) monophosphate. The THZ and HMP moieties are independently synthesized, and labeling studies have demonstrated probable metabolic precursors to both. We present herein the initial characterization of thiI, a gene required for THZ synthesis. We show that thiI is a 1,449-bp open reading frame located at minute 9.65 on the S. typhimurium chromosome and that it encodes a 483-amino-acid protein with a predicted molecular mass of 55 kDa. Unlike genes in the thiamine biosynthetic operon at minute 90, thiI is not transcriptionally regulated by TPP.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Base Sequence
  • Chromosome Mapping
  • Gene Expression Regulation, Bacterial
  • Genes, Bacterial*
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Polymerase Chain Reaction
  • Salmonella typhimurium / genetics*
  • Salmonella typhimurium / metabolism
  • Sequence Analysis, DNA
  • Sulfurtransferases*
  • Thiazoles / metabolism*
  • Transcription, Genetic


  • Bacterial Proteins
  • Thiazoles
  • 2-(4-methyl-1,3-thiazol-5-yl)ethanol
  • Sulfurtransferases
  • ThiI protein, bacteria

Associated data

  • GENBANK/U94901