HLA-B27 modulates intracellular survival of Salmonella enteritidis in human monocytic cells

Eur J Immunol. 1997 Jun;27(6):1331-8. doi: 10.1002/eji.1830270606.


Human major histocompatibility complex class I allele HLA-B27 is associated with a group of diseases called spondyloarthropathies. In reactive arthritis (ReA), the disease is triggered by certain infections, e.g. gastroenteritis caused by Salmonella. The host/microbe interaction is abnormal in susceptible individuals leading to inefficient elimination of arthritis-triggering bacteria, fragments of them, or both, after the initial infection. Using transfected human monocytic U937 cell lines, we demonstrate that the expression of the HLA-B27 antigen does not influence the uptake of S. enteritidis into U937 cells in vitro. Interestingly, HLA-B27 remarkably impairs the elimination of S. enteritidis within the HLA-B27 transfected U937 cells. The impaired elimination of ReA-triggering microbes by HLA-B27+ monocytes may offer an explanation for the persistence of ReA-triggering microbes in susceptible HLA-B27+ individuals. This modulation of the host/microbe interaction by HLA-B27 may have an important role in the pathogenesis of ReA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Count
  • Cell Line
  • Cell Survival / immunology
  • Gene Expression Regulation / immunology
  • Genes, MHC Class I / immunology
  • HLA-B27 Antigen / physiology*
  • Humans
  • Intracellular Fluid / immunology*
  • Intracellular Fluid / microbiology*
  • Microscopy, Electron
  • Microscopy, Fluorescence
  • Monocytes / immunology*
  • Monocytes / microbiology*
  • Monocytes / ultrastructure
  • Phagocytosis
  • Prohibitins
  • Salmonella enteritidis / growth & development
  • Salmonella enteritidis / immunology*
  • Salmonella enteritidis / ultrastructure
  • Transfection


  • HLA-B27 Antigen
  • PHB2 protein, human
  • Prohibitins