Evolution of RNA-binding specificity in T4 DNA polymerase

J Biol Chem. 1997 Jul 11;272(28):17703-10. doi: 10.1074/jbc.272.28.17703.


DNA polymerase of phage T4 (T4 gp43), an essential component of the T4 DNA replicase, is a multifunctional single-chained (898-amino acid) protein that catalyzes the highly accurate synthesis of DNA in phage replication. The enzyme functions both as a DNA-binding replication protein and as a sequence-specific RNA-binding autogenous translational repressor. We have utilized a phylogenetic approach to study the relationships between the two nucleic acid-binding functions of the protein. We found that autogenous translational control of gp43 biosynthesis has been conserved in phage RB69, a distant relative of T4, although we also found that the RB69 system differs from its T4 counterpart in two regards: (a) nucleotide sequence and predicted secondary structure of the RNA target (translational operator), and (b) RNA specificity of the protein. T4 gp43 is specific to the RNA operator sequence of the T4 genome whereas RB69 gp43 can bind and repress operator RNA from both phages equally well. In studies with T4-RB69 gp43 chimeras, we mapped T4 gp43 RNA-binding specificity to a protein segment that also harbors important determinants for DNA binding and the polymerase catalytic function. Our results suggest that RNA functions as a regulator of both the dosage and activity of this DNA replication enzyme.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacteriophage T4 / enzymology
  • Bacteriophage T4 / genetics
  • Base Sequence
  • Coliphages / enzymology
  • Coliphages / genetics
  • DNA, Viral / chemistry
  • DNA-Directed DNA Polymerase*
  • Evolution, Molecular*
  • Exonucleases / metabolism
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Operon
  • Protein Biosynthesis
  • RNA / metabolism*
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism*
  • Recombinant Fusion Proteins / metabolism
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism
  • Substrate Specificity
  • Viral Proteins / genetics
  • Viral Proteins / metabolism*


  • DNA, Viral
  • RNA-Binding Proteins
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • Viral Proteins
  • gene 43 protein, Enterobacteria phage T4
  • regA protein, bacteriophage RB69
  • RNA
  • DNA-Directed DNA Polymerase
  • Exonucleases