Human endothelial culture supernatant selectively promotes IgM-producing hybridomas

R Greferath; S Sauer-Nehls; S Dyballa; P Nehls

doi:10.1016/s0022-1759(97)00046-x

Human endothelial culture supernatant selectively promotes IgM-producing hybridomas

J Immunol Methods. 1997 May 26;204(2):189-92. doi: 10.1016/s0022-1759(97)00046-x.

Authors

R Greferath¹, S Sauer-Nehls, S Dyballa, P Nehls

Affiliation

¹ Center for Blood Research Laboratories, Harvard Medical School, Boston, MA 02135, USA.

PMID: 9212836
DOI: 10.1016/s0022-1759(97)00046-x

Abstract

We compared the effect of human endothelial cell culture supernatant (HECS), ESG (Ewing sarcoma growth factor), IL-6 (interleukin-6) and peritoneal macrophages on the recovery of hybridoma cells after fusion with respect to growth, stability and distribution of isotype variants. A selective growth of murine IgM-producing hybridoma cells was observed in the presence of HECS after cell fusion.

MeSH terms

Animals
Endothelium, Vascular / cytology*
Humans
Hybridomas / immunology*
Immunoglobulin G / biosynthesis
Immunoglobulin M / biosynthesis*
Interleukin-6 / pharmacology
Mice
Mice, Inbred BALB C

Substances

Immunoglobulin G
Immunoglobulin M
Interleukin-6