Reductive metabolism of 4-nitrobiphenyl by rat liver fraction

Carcinogenesis. 1997 Jun;18(6):1233-40. doi: 10.1093/carcin/18.6.1233.


The metabolites of 4-nitrobiphenyl (4-NBP) were studied using S-9 under anaerobic conditions. Ten metabolites were isolated and tentatively identified by high-performance liquid chromatography/atmospheric pressure chemical ionization/mass spectrometry (HPLC/APCI/MS), which is a novel efficient technqiue for analyses of metabolites. HPLC retention times and UV spectra have been also used for the confirmation. Among them, 4-aminobiphenyl (4-ABP, 79% of total metabolites) and hydroxylaminobiphenyls were found to be the major metabolites, and 4-acetylaminobiphenyl (4-AABP), N-hydroxy-4-acetylaminobiphenyl (4-AABP-N-OH), x-OH-4-nitrobiphenyl (4-NBP-x-OH), biphenylene and N-formyl-4-aminobiphenyl (N-formyl-4-ABP) were the minor metabolites. Based on the metabolities identified, different metabolic pathways of 4-NBP in rat liver, including N-hydroxylation, N-acetylation, N-formylation, de-nitro-group biotransformation and ring hydroxylation were proposed to elucidate the mechanisms of toxification and detoxification. In addition, experimental conditions such as incubation time and S-9 amounts were optimized.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromatography, High Pressure Liquid
  • Liver / metabolism*
  • Mass Spectrometry
  • Nitrophenols / metabolism*
  • Oxidation-Reduction
  • Rats
  • Serine Proteinase Inhibitors / metabolism*
  • Spectrophotometry, Ultraviolet


  • Nitrophenols
  • Serine Proteinase Inhibitors
  • 4-nitrophenyl