Induction of ABA 8'-hydroxylase by (+)-S-, (-)-R- and 8'-8'-8'-trifluoro-S-abscisic acid in suspension cultures of potato and Arabidopsis

Phytochemistry. 1997 Jul;45(5):931-4. doi: 10.1016/s0031-9422(97)00022-8.

Abstract

Suspension cultures of potato and Arabidopsis were incubated with 50 microM of (+)-ABA and (-)-ABA for 3 hr. These pretreatments were found to increase the rate, by two- to seven-fold, of formation of [2H6] phaseic acid (PA) from [2H6] ABA, applied in a subsequent incubation. Pretreatment with trifluoro-ABA had a higher efficacy, increasing the rate of conversion 15-fold. Suspension cell cultures that had been dehydrated and then rehydrated in the presence of [2H6] ABA displayed a much lower enhancement of PA formation. We conclude that ABA induces its own oxidative catabolism in suspension cultures.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Abscisic Acid / analogs & derivatives*
  • Abscisic Acid / metabolism
  • Arabidopsis / enzymology*
  • Cells, Cultured
  • Cytochrome P-450 Enzyme System / biosynthesis*
  • Desiccation
  • Deuterium
  • Enzyme Induction / drug effects
  • Mixed Function Oxygenases / biosynthesis*
  • Plant Proteins
  • Solanum tuberosum / enzymology*
  • Stereoisomerism

Substances

  • Plant Proteins
  • 8',8',8'-trifluoroabscisic acid
  • Abscisic Acid
  • Cytochrome P-450 Enzyme System
  • Deuterium
  • Mixed Function Oxygenases
  • abscisic acid 8'-hydroxylase