Effect of interferon-gamma and glucose on major histocompatibility complex class I and class II expression by pancreatic beta- and non-beta-cells

J Clin Endocrinol Metab. 1997 Jul;82(7):2329-36. doi: 10.1210/jcem.82.7.4055.


Surface major histocompatibility complex (MHC) class I and class II expression by pancreatic islet cells is considered a local initiator or regulator of immune processes that can lead to diabetes. Locally released cytokines, in particular interferon-gamma, are known to stimulate MHC antigen expression by islet cells. The present study quantifies MHC expression in cultured pancreatic beta- and non-beta-cells from both rat and human organs. Interferon-gamma increased MHC class I expression in endocrine beta- and non-beta-cells as well as in pancreatic ductal cells. The cytokine induced a 6-fold increase in the MHC class I messenger ribonucleic acid levels in pancreatic beta-cells; this effect was 2-fold amplified in the presence of elevated glucose levels (20 mmol/L instead of 6 mmol/L). No MHC class II expression was observed in endocrine beta- or non-beta-cells; human, but not rat, ductal cells exhibited MHC class II expression that increased in the presence of interferon-gamma. These data indicate that the increase in beta-cell MHC class I expression described in the pancreata of diabetic patients may result from stimulated transcription after exposure to locally released interferon-gamma and/or to a hyperglycemic state. The association of human islets with ductal cells in which MHC class II expression is stimulated by interferon-gamma makes these cells potential participants in the autoimmune process in diabetes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Animals
  • Flow Cytometry
  • Fluorescent Antibody Technique, Indirect
  • Glucose / pharmacology*
  • Histocompatibility Antigens Class I / metabolism*
  • Histocompatibility Antigens Class II / metabolism*
  • Humans
  • Immunohistochemistry
  • Interferon-gamma / pharmacology*
  • Islets of Langerhans / drug effects
  • Islets of Langerhans / metabolism*
  • Keratins / metabolism
  • Male
  • Middle Aged
  • Pancreas / drug effects
  • Pancreas / metabolism*
  • Rats
  • Rats, Wistar
  • beta 2-Microglobulin / metabolism


  • Histocompatibility Antigens Class I
  • Histocompatibility Antigens Class II
  • beta 2-Microglobulin
  • Keratins
  • Interferon-gamma
  • Glucose