Expression of estrogen receptor-beta in human breast tumors

J Clin Endocrinol Metab. 1997 Jul;82(7):2371-4. doi: 10.1210/jcem.82.7.4212.


The expression of a recently described novel estrogen receptor, ER-beta, was detected in several human breast tumor biopsy samples and several human breast epithelial cell lines using reverse transcription and polymerase chain reaction (RT-PCR) analysis. Cloning and sequencing of the PCR product from a breast tumor confirmed the identity of the sequence with that of the ER-beta mRNA previously reported in human testis. The expression of ER-beta was not correlated with that of ER-alpha, and both ER-alpha positive and ER-alpha negative cell lines expressed ER-beta mRNA. However, some breast tumors and some cell lines coexpress ER-beta and ER-alpha mRNA. Our data support a possible role for ER-beta in human breast cancer.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biopsy
  • Breast Neoplasms / metabolism*
  • Cells, Cultured
  • Estrogen Receptor beta
  • Female
  • Humans
  • Male
  • Polymerase Chain Reaction
  • RNA, Messenger / metabolism
  • Receptors, Estrogen / metabolism*
  • Testis / metabolism
  • Tumor Cells, Cultured


  • Estrogen Receptor beta
  • RNA, Messenger
  • Receptors, Estrogen