14-3-3 proteins are essential for RAS/MAPK cascade signaling during pseudohyphal development in S. cerevisiae

Cell. 1997 Jun 27;89(7):1055-65. doi: 10.1016/s0092-8674(00)80293-7.

Abstract

14-3-3 proteins are highly conserved ubiquitous proteins whose explicit functions have remained elusive. Here, we show that the S. cerevisiae 14-3-3 homologs BMH1 and BMH2 are not essential for viability or mating MAPK cascade signaling, but they are essential for pseudohyphal-development MAPK cascade signaling and other processes. Activated alleles of RAS2 and CDC42 induce pseudohyphal development and FG(TyA)-lacZ signaling in Bmh+ strains but not in ste20 (p65PAK) or bmh1 bmh2 mutant strains. Moreover, Bmh1p and Bmh2p associate with Ste20p in vivo. Three alleles of BMH1 encode proteins defective for FG(TyA)-lacZ signaling and association with Ste20p, yet these alleles complement other 14-3-3 functions. Therefore, the 14-3-3 proteins are specifically required for RAS/MAPK cascade signaling during pseudohyphal development in S. cerevisiae.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 14-3-3 Proteins
  • Adaptation, Physiological
  • Alleles
  • Biological Evolution
  • Cell Survival / physiology
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • GTP-Binding Proteins / metabolism
  • Glycogen / metabolism
  • Intracellular Signaling Peptides and Proteins
  • Lac Operon / genetics
  • MAP Kinase Kinase Kinases
  • Molecular Sequence Data
  • Mutagenesis / physiology
  • Phenotype
  • Protein Structure, Tertiary
  • Protein-Serine-Threonine Kinases / metabolism
  • Recombinant Proteins / genetics
  • Reproduction / physiology
  • Saccharomyces cerevisiae / chemistry
  • Saccharomyces cerevisiae / cytology*
  • Saccharomyces cerevisiae / ultrastructure
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid
  • Signal Transduction / physiology*
  • Species Specificity
  • ras Proteins / physiology*

Substances

  • 14-3-3 Proteins
  • BMH1 protein, S cerevisiae
  • BMH2 protein, S cerevisiae
  • Fungal Proteins
  • Intracellular Signaling Peptides and Proteins
  • Recombinant Proteins
  • Saccharomyces cerevisiae Proteins
  • Glycogen
  • Protein-Serine-Threonine Kinases
  • MAP Kinase Kinase Kinases
  • STE20 protein, S cerevisiae
  • GTP-Binding Proteins
  • ras Proteins