Characterization of two newly established cell lines derived from squamous cell carcinomas of the oesophagus

Anticancer Res. May-Jun 1997;17(3C):2185-92.

Abstract

This study describes characteristics of two newly established cell lines (OSC-1 and OSC-2), derived from two oesophageal squamous cell carcinomas. Morphologically, OSC-1 cells and OSC-2 cells grew in epithelial cobblestone patterns with cells piling up to 4 cells. Ultrastructurally, both cell lines showed formation of desmosomes; however, tonofilaments were only formed by OSC-2 cells. Immunohistochemical investigations revealed coexpression of vimentin and cytokeratin in OSC-1 cells and OSC-2 cells. A cytokeratin subtype typical for mature squamous epithelia (cytokeratin 13) was expressed only in OSC-2 cells. OSC-1 cells showed tumour formation in nude mice, whereas OSC-2 cells did not. Cytogenetic analysis revealed that OSC-1 cells had a hyperdiploid karyotype and OSC-2 cells had a near-triploid karyotype. In both cell lines, the formation of multicellular spheroids could be induced. In conclusion, in comparison with OSC-2 cells the OSC-1 cells were characterized by a poorer degree of differentiation and by a more aggressive growth behaviour in vivo.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Carcinoma, Squamous Cell / genetics
  • Carcinoma, Squamous Cell / pathology*
  • Carcinoma, Squamous Cell / ultrastructure
  • Cell Culture Techniques / methods
  • Cell Cycle
  • Cell Line
  • Chromosome Banding
  • Desmosomes / pathology
  • Desmosomes / ultrastructure
  • Esophageal Neoplasms / genetics
  • Esophageal Neoplasms / pathology*
  • Esophageal Neoplasms / ultrastructure
  • Humans
  • Immunohistochemistry
  • Karyotyping
  • Keratins / analysis
  • Keratins / biosynthesis
  • Metaphase
  • Mice
  • Mice, Nude
  • Microscopy, Electron
  • Microscopy, Phase-Contrast
  • Transplantation, Heterologous
  • Tumor Cells, Cultured
  • Vimentin / analysis
  • Vimentin / biosynthesis

Substances

  • Vimentin
  • Keratins