Cloning and sequencing of the cellular-viral junctions from the human adenovirus type 5 transformed 293 cell line

Virology. 1997 Jul 7;233(2):423-9. doi: 10.1006/viro.1997.8597.

Abstract

The human-viral junctions of integrated adenovirus type 5 (Ad5) DNA in 293 cells have been cloned and sequenced. The Ad5 sequences extend from nucleotides (nt) 1 to 4344 and are located in the pregnancy-specific beta-1-glycoprotein 4 (PSG 4) gene. This maps the insertion of Ad5 DNA to human chromosome 19 (19q13.2). The Ad5 sequences are represented as a single collinear insertion of viral DNA with no rearrangements. There were 19 bp of PSG4 DNA deleted at the site of insertion and an extra 3 nt (GTC) at the left viral/cellular junction. A short patch of homology between viral DNA and PSG4 DNA (6 nt with 1 mismatch) was present at the right junction.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoviruses, Human / genetics*
  • Base Sequence
  • Binding Sites
  • Blotting, Southern
  • Cell Line, Transformed
  • Chromosomes, Human, Pair 19
  • Cloning, Molecular
  • DNA, Viral / genetics
  • Genetic Vectors
  • Humans
  • Molecular Sequence Data
  • Virus Integration*

Substances

  • DNA, Viral