Roles of N-glycans with alpha2,6 as well as alpha2,3 linked sialic acid in infection by polyoma virus

Virology. 1997 Jul 7;233(2):440-2. doi: 10.1006/viro.1997.8596.


Specific glycosylation inhibitors were used to show that N-glycans, and not O-glycans or glycolipids, constitute the major class of receptors on 3T3 cells for polyoma virus. Sialic acid (SA)-specific lectins were used in attempts to confirm the expected SA linkage on N-linked glycans. A lectin specific for alpha2,6 linked SA, a non-receptor type, was slightly more effective in blocking infection than a lectin specific for alpha2,3 linked SA which corresponds to the known receptor specificity. Possible explanations are suggested for this unexpected result.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 1-Deoxynojirimycin / pharmacology
  • 3T3 Cells
  • Animals
  • Lectins / pharmacology
  • Mice
  • N-Acetylneuraminic Acid / physiology*
  • Polyomavirus / pathogenicity*
  • Polysaccharides / physiology*
  • Receptors, Cell Surface
  • Receptors, Virus / physiology*
  • Tunicamycin / pharmacology


  • Lectins
  • Polysaccharides
  • Receptors, Cell Surface
  • Receptors, Virus
  • sialic acid receptor
  • Tunicamycin
  • 1-Deoxynojirimycin
  • N-Acetylneuraminic Acid