Regulation of E-selectin, P-selectin, and intercellular adhesion molecule 1 expression in mouse cremaster muscle vasculature

Microcirculation. 1997 Jun;4(2):311-9. doi: 10.3109/10739689709146794.


Objective: To investigate the expression of P- and E-selectin and intercellular adhesion molecule 1 (ICAM-1) in the vasculature of mouse cremaster muscles both with and without tumor necrosis factor alpha (TNF-alpha) treatment.

Methods: Mice received injections of monoclonal antibody to P-selectin, E-selectin, or ICAM-1 before fixation to restrict detection to antigen expressed on the endothelial surface. Whole-mount preparations of mouse cremaster muscles were fixed in acetone and stained using biotinylated secondary antibody and peroxidase-conjugated streptavidin.

Results: P-selectin is expressed on the endothelial surface of cremaster muscle venules within 10 minutes after exteriorization. Expression increases upon treatment with TNF-alpha (2 hours), reflecting transcriptional regulation of P-selectin expression in situ. The baseline E-selectin expression is patchy and barely detectable but shows a significant upregulation after treatment with TNF-alpha. [CAM-1 is constitutively expressed in unstimulated mouse cremaster venules and slightly upregulated after 2 hours of TNF-alpha treatment. Under baseline conditions, neither E-selectin, P-selectin, nor ICAM-1 is detectable in arterioles or capillaries. After TNF-alpha treatment, arterioles stain faintly for P-selectin, but not E-selectin or ICAM-1.

Conclusion: The temporal and spatial pattern of expression of P- and E-selectin and ICAM-1 is consistent with the functional role of these molecules in mediating preferential leukocyte rolling and adhesion in mouse cremaster muscle venules.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / chemistry
  • Arterioles / immunology
  • Arterioles / metabolism
  • E-Selectin / drug effects*
  • E-Selectin / immunology
  • E-Selectin / metabolism*
  • Immunohistochemistry
  • In Vitro Techniques
  • Intercellular Adhesion Molecule-1 / drug effects*
  • Intercellular Adhesion Molecule-1 / immunology
  • Intercellular Adhesion Molecule-1 / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Muscle, Skeletal / blood supply*
  • Muscle, Skeletal / drug effects*
  • Muscle, Skeletal / surgery
  • P-Selectin / drug effects*
  • P-Selectin / immunology
  • P-Selectin / metabolism*
  • Tumor Necrosis Factor-alpha / pharmacology
  • Venules / immunology
  • Venules / metabolism


  • Antibodies, Monoclonal
  • E-Selectin
  • P-Selectin
  • Tumor Necrosis Factor-alpha
  • Intercellular Adhesion Molecule-1