Cloning of the mouse laminin alpha 4 cDNA. Expression in a subset of endothelium

Eur J Biochem. 1997 Jun 15;246(3):727-35. doi: 10.1111/j.1432-1033.1997.t01-1-00727.x.

Abstract

Endothelial cells express a 400-kDa or 240-kDa laminin alpha chain, depending on their tissue of origin or physiological state [1, 2]. Using differential display and subsequent screening of a mouse endothelial cell cDNA library we here identify the gene coding for the 240-kDa laminin chain as the laminin alpha 4 gene. The complete mouse laminin alpha 4 cDNA sequence is reported and compared with other laminin alpha chains. In situ hybridization of embryonic and new born mouse tissues revealed expression of laminin alpha 4 mRNA in a subset of endothelium, in particular aortic endothelium, endocardium and endothelium of blood vessels in the skin and in the brain. Strong laminin alpha 4 expression by aortic endothelia was confirmed by data obtained from cultured bovine aortic endothelial cells (BAEC). Isolation of laminin from BAEC conditioned medium revealed a Y-shaped molecule in rotary shadowing. Subsequent sequencing of BAEC laminin resulted in laminin alpha 4, beta 1 and gamma 1 amino acid sequences, confirming that laminin alpha 4 is one of the major laminin alpha chains expressed by aortic endothelium not only in the mouse. In addition, strong laminin alpha 4 mRNA expression occurred in peripheral nerves, cardiac muscle, fat, the dermis of the skin and lung stroma of mouse tissues. The data demonstrate a cytokine and progesterone-regulated differential expression of laminin alpha 4 mRNA in mouse endothelium, suggesting a distinct functional role for this laminin chain in endothelium.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cattle
  • Cloning, Molecular
  • Cytokines / pharmacology
  • DNA, Complementary / metabolism
  • Endothelium, Vascular / metabolism*
  • Gene Expression / drug effects
  • In Situ Hybridization
  • Laminin / genetics*
  • Mice
  • Molecular Sequence Data
  • Progesterone / pharmacology
  • RNA, Messenger / metabolism

Substances

  • Cytokines
  • DNA, Complementary
  • Lama4 protein, mouse
  • Laminin
  • RNA, Messenger
  • Progesterone

Associated data

  • GENBANK/Y09827