Regulation of phospholipases C and D in rat ventricular myocytes: stimulation by endothelin-1, bradykinin and phenylephrine

J Mol Cell Cardiol. 1997 Jun;29(6):1593-604. doi: 10.1006/jmcc.1997.0395.


The physiological activator of protein kinase C (PKC), diacylglycerol, is formed by hydrolysis of phosphoinositides (PI) by phospholipase C (PLC) or phosphatidylcholine by phospholipase D (PLD). We have measured activation of these phospholipases by endothelin-1 (ET-1), bradykinin (BK), or phenylephrine (PE) in ventricular myocytes cultured from neonatal rat. The stimulation of PI hydrolysis after 10 min by 0.1 microM ET-1 (about 12-fold) was much greater than for BK or PE (each about four-fold), and did not correlate with translocation of nPKC delta or nPKC epsilon (Clerk A. Bogoyevitch MA. Andersson MB. Sugden PH, 1994. J Biol Chem 269: 32848-32857: Clerk A, Gillespie-Brown J, Fuller SJ, Sugden PH, 1996. Biochem J 317: 109-118). However, ET-1 and BK stimulated a similar rapid increase in [3H]InsP, formation (< 30 s), which was much greater than that seen with PE. This early phase correlated with PKC translocation. Acute or chronic exposure to 12-O-tetradecanoylphorbol-13-acetate (TPA) or treatment with Ro-31-8220 showed that the stimulation of PI hydrolysis by PE, but not ET-1 or BK, was inhibited by activation of PKC. Furthermore, ET-1 and BK heterologously desensitized the stimulation of PI hydrolysis by PE, ET-1 or BK homologously uncoupled their own receptors from [3H]InsP3 formation, but there was no evidence of heterologous desensitization with these two agonists. Anomalously, chronic exposure to TPA increased the stimulation of PI hydrolysis by BK, but this probably resulted from an increase in BK receptor density. PLD was also rapidly activated by TPA. ET-1, BK or PE. Experiments with Ro-31-8220 showed that the stimulation of PLD by ET-1 and BK was mediated through activation of PKC. We discuss the characteristics of the activation of PI hydrolysis and PLD by ET-1, BK, and PE with respect to the translocation of PKC.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenergic alpha-Agonists / pharmacology
  • Animals
  • Bradykinin / pharmacology*
  • Cells, Cultured
  • Endothelin-1 / pharmacology*
  • Heart Ventricles / cytology
  • Heart Ventricles / drug effects
  • Heart Ventricles / enzymology*
  • Hydrolysis
  • Phenylephrine / pharmacology
  • Phosphatidylinositols / metabolism
  • Phospholipase D / drug effects
  • Phospholipase D / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Adrenergic, alpha / drug effects
  • Receptors, Adrenergic, alpha / metabolism
  • Tetradecanoylphorbol Acetate / pharmacology
  • Type C Phospholipases / drug effects
  • Type C Phospholipases / metabolism*


  • Adrenergic alpha-Agonists
  • Endothelin-1
  • Phosphatidylinositols
  • Receptors, Adrenergic, alpha
  • Phenylephrine
  • Type C Phospholipases
  • Phospholipase D
  • Tetradecanoylphorbol Acetate
  • Bradykinin