Blood flow patterns in the rat pancreas: a simulative demonstration by injection replication and scanning electron microscopy

Microsc Res Tech. 1997 Jun 1-15;37(5-6):497-508. doi: 10.1002/(SICI)1097-0029(19970601)37:5/6<497::AID-JEMT12>3.0.CO;2-L.

Abstract

Scanning electron microscopy of vascular casts prepared by arterial injections of intentionally reduced amounts of resin showed that in the rat pancreas, the casting medium fills blood capillaries in the endocrine islets more promptly than those in the exocrine lobules and secretory ducts. Furthermore, the exocrine lobules containing endocrine islets allowed a more rapid resin flow through the insulo-acinar portal route than those lobules lacking an islet. The capillaries of secretory ducts were the last portions to be filled with resin. Since the resin used in this study was as viscous as blood and injected under a physiological pressure, the microcirculatory patterns demonstrated by the present method reflect the physiological flow pattern of blood in the pancreas.

MeSH terms

  • Animals
  • Arteries / anatomy & histology
  • Arteries / ultrastructure
  • Blood Circulation*
  • Capillaries / anatomy & histology
  • Capillaries / ultrastructure
  • Exocrine Glands / blood supply
  • Exocrine Glands / physiology
  • Exocrine Glands / ultrastructure
  • Islets of Langerhans / blood supply
  • Islets of Langerhans / physiology
  • Islets of Langerhans / ultrastructure
  • Male
  • Microcirculation / physiology
  • Microscopy, Electron, Scanning / methods
  • Pancreas / blood supply*
  • Pancreas / physiology
  • Pancreas / ultrastructure*
  • Pancreatic Ducts / blood supply
  • Pancreatic Ducts / ultrastructure
  • Portal System / physiology
  • Portal System / ultrastructure
  • Rats
  • Rats, Wistar / anatomy & histology*