Nicotinic acetylcholine receptor antagonist effect of fluoxetine in rat hippocampal slices

Brain Res. 1997 Jun 13;759(2):292-4. doi: 10.1016/s0006-8993(97)00343-0.


We compared the effect of mecamylamine and fluoxetine on the hippocampal noradrenaline (NA) release evoked by nicotine in vitro. Nicotine (100 microM) increased the basal release of [3H]NA from rat hippocampal slices. This effect was blocked by the potent nicotinic antagonist mecamylamine in a dose-dependent manner (IC50 = 0.19 microM). The selective serotonin reuptake inhibitor (SSRI) fluoxetine also antagonised the response to nicotine in a dose-dependent manner with a similar strength (IC50 = 0.57 microM). Our data indicate that fluoxetine has nicotinic acetylcholine receptor antagonist effect in the central nervous system. The possible clinical significance of this finding is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Dose-Response Relationship, Drug
  • Fluoxetine / pharmacology*
  • Hippocampus / drug effects*
  • Male
  • Mecamylamine / pharmacology
  • Nicotinic Antagonists / pharmacology*
  • Rats
  • Rats, Wistar


  • Nicotinic Antagonists
  • Fluoxetine
  • Mecamylamine