Inhibition by heterologously-expressed P2Y2 nucleotide receptors of N-type calcium currents in rat sympathetic neurones

Br J Pharmacol. 1997 Jul;121(5):849-51. doi: 10.1038/sj.bjp.0701270.

Abstract

The P2Y2 nucleotide receptor has previously been shown to stimulate phosphoinositide breakdown. We now show that, when P2Y2 receptors are heterologously expressed by cRNA injection into dissociated rat sympathetic neurones, activation of these receptors by uridine 5'-triphosphate (UTP) or adenosine 5'-triphosphate (ATP) inhibits the N-type voltage-gated calcium current by approximately 65%, with an IC50 of 0.5 microM. Thus, the same molecular species of nucleotide receptor can link to two different effector pathways.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium Channels / metabolism*
  • Ion Channel Gating / physiology
  • Neurons / metabolism*
  • Phosphatidylinositols / metabolism
  • Purinergic P2 Receptor Antagonists*
  • RNA, Complementary / metabolism
  • Rats
  • Sympathetic Nervous System / cytology
  • Sympathetic Nervous System / metabolism*

Substances

  • Calcium Channels
  • Phosphatidylinositols
  • Purinergic P2 Receptor Antagonists
  • RNA, Complementary