Sorbitol accumulation and transmembrane efflux in osmotically stressed JS1 schwannoma cells

Neurosci Lett. 1997 Jun 20;229(1):53-6. doi: 10.1016/s0304-3940(97)00416-3.

Abstract

Sorbitol accumulation and transmembrane efflux in JS1 schwannoma cells were examined during osmotic stress in the presence of a sorbitol dehydrogenase (SD) inhibitor and following return to iso-osmotic conditions. SD inhibition promoted sorbitol accumulation under hyperglycemic and/or hyperosmotic conditions, and sorbitol efflux during iso-osmotic incubation was prevented by cooling to 4 degrees C or by quinidine. It appears that sorbitol levels in JS1 cells are dependent on SD activity and that sorbitol is rapidly removed upon restoring iso-osmotic conditions.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Brain Neoplasms / metabolism*
  • Cell Membrane / metabolism
  • Culture Media
  • L-Iditol 2-Dehydrogenase / antagonists & inhibitors
  • L-Iditol 2-Dehydrogenase / metabolism
  • Neurilemmoma / metabolism*
  • Osmotic Pressure
  • Polymers / metabolism
  • Quinidine / pharmacology
  • Rats
  • Sorbitol / metabolism*
  • Temperature
  • Tumor Cells, Cultured

Substances

  • Culture Media
  • Polymers
  • polyol
  • Sorbitol
  • L-Iditol 2-Dehydrogenase
  • Quinidine