FOG, a Multitype Zinc Finger Protein, Acts as a Cofactor for Transcription Factor GATA-1 in Erythroid and Megakaryocytic Differentiation

Cell. 1997 Jul 11;90(1):109-19. doi: 10.1016/s0092-8674(00)80318-9.

Abstract

The hematopoietic transcription factor GATA-1 is essential for development of the erythroid and megakaryocytic lineages. Using the conserved zinc finger DNA-binding domain of GATA-1 in the yeast two-hybrid system, we have identified a novel, multitype zinc finger protein, Friend of GATA-1 (FOG), which binds GATA-1 but not a functionally inactive mutant lacking the amino (N) finger. FOG is coexpressed with GATA-1 during embryonic development and in erythroid and megakaryocytic cells. Furthermore, FOG and GATA-1 synergistically activate transcription from a hematopoietic-specific regulatory region and cooperate during both erythroid and megakaryocytic cell differentiation. These findings indicate that FOG acts as a cofactor for GATA-1 and provide a paradigm for the regulation of cell type-specific gene expression by GATA transcription factors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells
  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • Carrier Proteins / biosynthesis*
  • Carrier Proteins / chemistry
  • Cell Differentiation / physiology*
  • Cloning, Molecular
  • Conserved Sequence
  • DNA-Binding Proteins / biosynthesis*
  • DNA-Binding Proteins / chemistry
  • Embryo, Mammalian
  • Embryonic and Fetal Development
  • Erythroid Precursor Cells / cytology*
  • Erythroid Precursor Cells / physiology*
  • Erythroid-Specific DNA-Binding Factors
  • GATA1 Transcription Factor
  • Gene Expression Regulation, Developmental*
  • Hematopoiesis
  • Megakaryocytes / cytology*
  • Megakaryocytes / physiology*
  • Mice
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Nuclear Proteins / biosynthesis*
  • Nuclear Proteins / chemistry
  • Polymerase Chain Reaction
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / chemistry
  • Saccharomyces cerevisiae
  • Transcription Factors / biosynthesis*
  • Transcription Factors / chemistry
  • Transcriptional Activation
  • Transfection
  • Zinc Fingers*

Substances

  • Carrier Proteins
  • DNA-Binding Proteins
  • Erythroid-Specific DNA-Binding Factors
  • GATA1 Transcription Factor
  • Gata1 protein, mouse
  • Nuclear Proteins
  • Recombinant Fusion Proteins
  • Transcription Factors
  • Zfpm1 protein, mouse

Associated data

  • GENBANK/AF006492