ACF, an ISWI-containing and ATP-utilizing chromatin assembly and remodeling factor

Cell. 1997 Jul 11;90(1):145-55. doi: 10.1016/s0092-8674(00)80321-9.


We describe the purification and characterization of ACF, an ATP-utilizing chromatin assembly and remodeling factor. ACF is a multisubunit factor that contains ISWI protein and is distinct from NURF, another ISWI-containing factor. In chromatin assembly, purified ACF and a core histone chaperone (such as NAP-1 or CAF-1) are sufficient for the ATP-dependent formation of periodic nucleosome arrays. In chromatin remodeling, ACF is able to modulate the internucleosomal spacing of chromatin by an ATP-dependent mechanism. Moreover, ACF can mediate promoter-specific nucleosome reconfiguration by Gal4-VP16 in an ATP-dependent manner. These results suggest that ACF acts catalytically both in chromatin assembly and in the remodeling of nucleosomes that occurs during transcriptional activation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphatases / chemistry
  • Adenosine Triphosphatases / isolation & purification
  • Adenosine Triphosphatases / metabolism*
  • Adenosine Triphosphate / metabolism*
  • Animals
  • Chromatin / physiology*
  • Drosophila / embryology
  • Drosophila / physiology
  • Embryo, Nonmammalian / physiology
  • Macromolecular Substances
  • Nucleosomes / physiology
  • Promoter Regions, Genetic
  • Transcription Factors / chemistry
  • Transcription Factors / isolation & purification
  • Transcription Factors / metabolism*
  • Transcription, Genetic


  • Chromatin
  • ISWI protein
  • Macromolecular Substances
  • Nucleosomes
  • Transcription Factors
  • Adenosine Triphosphate
  • Adenosine Triphosphatases