A Plasmid-Mediated CMY-2 Beta-Lactamase From an Algerian Clinical Isolate of Salmonella Senftenberg

FEMS Microbiol Lett. 1997 Jul 15;152(2):255-60. doi: 10.1111/j.1574-6968.1997.tb10436.x.

Abstract

Multiresistance to antibiotics including beta-lactams, e.g. cefoxitin, was transferred by conjugation to Escherichia coli strain C1a from a clinical isolate of Salmonella senftenberg recovered from stools of an Algerian child. The susceptibility pattern to beta-lactams was similar to the profile mediated by an AmpC-type beta-lactamase. By biochemical analysis, typical AmpC-type enzyme substrate and inhibition profiles were obtained. Finally, an ampC plasmid-encoded beta-lactamase gene was cloned and sequenced. Its deduced amino acid sequence confirmed its identity as a class C beta-lactamase. It showed 99.5% sequence identity with the plasmid-mediated beta-lactamase CMY-2. The differences in the amino acid sequences of the two enzymes were located in the signal peptide.

MeSH terms

  • Algeria
  • Anti-Bacterial Agents / pharmacology
  • Child
  • Drug Resistance, Multiple / genetics*
  • Feces / microbiology
  • Humans
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Plasmids / genetics*
  • Salmonella / drug effects*
  • Salmonella / enzymology
  • Salmonella / genetics
  • Salmonella / isolation & purification
  • beta-Lactam Resistance / genetics*
  • beta-Lactamases / genetics*
  • beta-Lactams / pharmacology

Substances

  • Anti-Bacterial Agents
  • beta-Lactams
  • beta-Lactamases

Associated data

  • GENBANK/U77414