Establishment of an experimental intrapulmonary tumor nodule model

Ann Thorac Surg. 1997 Jul;64(1):216-9. doi: 10.1016/s0003-4975(97)00343-3.


Background: A pulmonary tumor model is necessary to study the biology and therapy of lung cancer. Methods to establish a solitary intrapulmonary nodule are not well defined. Two methods for solitary intrapulmonary tumor nodule development in the Fischer rat are described.

Methods: Methylcholanthrene-induced sarcoma cell suspensions were introduced into lung parenchyma of Fischer rats via limited thoracotomy and lung puncture, or instilled into a distal airway after tracheal puncture and catheterization. Intrapulmonary tumor location, implantation mortality, procedure length, and animal survival were recorded.

Results: Single pulmonary nodules developed at the implanted position in 100% (n = 320) and 95% (62/65) of animals after direct injection into the pulmonary parenchyma or via tracheal puncture and instillation. Operative mortality was 2% and 5% via lung or tracheal implantation, respectively. Less than 5 minutes was required for each implantation. Mean survival time was 24 +/- 2 and 26 +/- 6 days after lung or tracheal implantation in animals allowed to survive until tumor-induced death.

Conclusions: These easily performed, reproducible methods of establishing solitary intrapulmonary tumors are useful tools for lung cancer research.

MeSH terms

  • Animals
  • Disease Models, Animal*
  • Lung Neoplasms* / pathology
  • Neoplasm Transplantation
  • Rats
  • Rats, Inbred F344
  • Sarcoma, Experimental* / pathology
  • Solitary Pulmonary Nodule* / pathology
  • Tumor Cells, Cultured