The Schizosaccharomyces pombe mam1 gene encodes an ABC transporter mediating secretion of M-factor

Mol Gen Genet. 1997 Jun;255(2):226-36. doi: 10.1007/s004380050493.

Abstract

In the fission yeast Schizosaccharomyces pombe, cells of opposite mating type communicate via diffusible peptide pheromones prior to mating. We have cloned the S. pombe mam1 gene, which encodes a 1336-amino acid protein belonging to the ATP-binding cassette (ABC) superfamily. The mam1 gene is only expressed in M cells and the gene product is responsible for the secretion of the mating pheromone. M-factor, a nonapeptide that is S-farnesylated and carboxy-methylated on its C-terminal cysteine residue. The predicted Mam1 protein is highly homologous to mammalian multiple drug-resistance proteins and to the Saccharomyces cerevisiae STE6 gene product, which mediates export of a-factor mating pheromone. We show that STE6 can also mediate secretion of M-factor in S. pombe.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics
  • ATP-Binding Cassette Transporters / genetics*
  • ATP-Binding Cassette Transporters / metabolism*
  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA-Binding Proteins
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Gene Expression Regulation, Fungal
  • Genetic Complementation Test
  • Glycoproteins*
  • Guanine Nucleotide Exchange Factors*
  • Intercellular Signaling Peptides and Proteins
  • Molecular Sequence Data
  • Mutation
  • Nitrogen / metabolism
  • Peptides / metabolism*
  • Pheromones / metabolism
  • Saccharomyces cerevisiae Proteins*
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces pombe Proteins*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Transcription, Genetic

Substances

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • ATP-Binding Cassette Transporters
  • DNA-Binding Proteins
  • Fungal Proteins
  • Glycoproteins
  • Guanine Nucleotide Exchange Factors
  • Intercellular Signaling Peptides and Proteins
  • Mating Factor, S pombe
  • Peptides
  • Pheromones
  • STE6 protein, S cerevisiae
  • STE6 protein, S pombe
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces pombe Proteins
  • mam1 protein, S pombe
  • mfm1 protein, S pombe
  • Nitrogen

Associated data

  • GENBANK/U66305