Utilization of hepatocyte-specific antibody in the immunocytochemical evaluation of liver tumors

Mod Pathol. 1997 Jul;10(7):686-92.


A monoclonal antibody highly specific for benign and malignant hepatocytes (HepPar 1) was evaluated as part of an antibody panel used to differentiate hepatocellular from nonhepatocellular neoplasms. Sixty-five liver tumors and two extrahepatic tumors from patients with documented liver tumors were studied. Twenty-two neoplasms were of hepatocellular origin, three were combined hepatocellular/cholangiocarcinomas, and the remainder were of nonhepatocellular origin. HepPar 1 alone had an 82% sensitivity and 90% specificity for the detection of hepatocellular neoplasms. The corresponding values for alpha-fetoprotein were 57% and 97%. Polyclonal antibody to carcinoembryonic antigen (canalicular pattern) had a sensitivity of 79% and specificity of 97% for these tumors. The use of antibody panels provided superior results when compared with individual antibodies. In summary, HepPar 1 monoclonal antibody is a useful reagent for the differential diagnosis of hepatocellular tumors. Its utility is enhanced when it is used as part of a diagnostic antibody panel.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Antibodies, Monoclonal*
  • Carcinoembryonic Antigen / analysis
  • Carcinoma, Hepatocellular / diagnosis*
  • Evaluation Studies as Topic
  • Female
  • Humans
  • Immunohistochemistry
  • Infant
  • Liver / chemistry
  • Liver / immunology*
  • Liver Neoplasms / diagnosis*
  • Male
  • Middle Aged
  • Sensitivity and Specificity
  • alpha-Fetoproteins / analysis


  • Antibodies, Monoclonal
  • Carcinoembryonic Antigen
  • alpha-Fetoproteins