In vivo inhibition of veratridine-evoked release of striatal excitatory amino acids by the group II metabotropic glutamate receptor agonist LY354740 in rats

Neurosci Lett. 1997 Jul 4;229(3):161-4. doi: 10.1016/s0304-3940(97)00442-4.


In vivo microdialysis in freely moving rats was used to investigate the presynaptic mechanisms by which LY354740, a novel, potent, selective, and systemically active agonist for group II metabotropic glutamate receptors (mGluRs), alters glutamate neuronal transmission. Basal levels of glutamate and aspartate in striatal dialysates of LY354740 (10 mg/kg i.p.)-treated animals were not significantly different from the saline-treated control animals. In the saline treated controls, veratridine (100 microM) induced a 6-fold increase in glutamate and 9-fold increase in aspartate. However, following LY354740 administration the veratridine-evoked release of glutamate and aspartate was completely prevented. These data demonstrate that LY354740 blocks the evoked release of endogenous excitatory amino acids, and indicate a role for group II mGluRs in presynaptic modulation of glutamate neuronal transmission in vivo. Ireland Ltd.

MeSH terms

  • Animals
  • Aspartic Acid / analysis
  • Aspartic Acid / metabolism
  • Bridged Bicyclo Compounds / analysis
  • Bridged Bicyclo Compounds / pharmacology*
  • Corpus Striatum / chemistry*
  • Corpus Striatum / drug effects
  • Excitatory Amino Acid Agonists / analysis
  • Excitatory Amino Acid Agonists / pharmacology*
  • Glutamic Acid / analysis
  • Glutamic Acid / metabolism
  • Male
  • Microdialysis
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Metabotropic Glutamate / agonists
  • Receptors, Metabotropic Glutamate / metabolism*
  • Synaptic Transmission / drug effects
  • Veratridine / pharmacology


  • Bridged Bicyclo Compounds
  • Excitatory Amino Acid Agonists
  • Receptors, Metabotropic Glutamate
  • Aspartic Acid
  • Glutamic Acid
  • Veratridine
  • eglumetad