Abstract
The effect of nitric oxide (NO) on osteoblastic differentiation was examined in cultured mouse osteoblasts. Interleukin-1beta and tumor necrosis factor-alpha expressed inducible NO synthase gene with little effect on constitutive NO synthase gene. These cytokines increased NO production, which was inhibited by L-NMMA pretreatment, and decreased alkaline phosphatase (AIPase) activity, which was not restored by L-NMMA. Furthermore, NO donors, sodium nitroprusside and NONOate dose-dependently elevated AIPase activity and expression of osteocalcin gene. These results suggest that NO directly facilitates osteoblastic differentiation and the cytokine-induced inhibition of AIPase activity is mediated via mechanism other than NO.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaline Phosphatase / biosynthesis
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Animals
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Cell Differentiation / drug effects
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Cells, Cultured
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Cyclic GMP / biosynthesis
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Dinoprostone / biosynthesis
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Hydrazines / pharmacology
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Interleukin-1 / pharmacology
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Mice
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Nitric Oxide / pharmacology*
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Nitric Oxide Synthase / genetics
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Nitrogen Oxides
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Nitroprusside / pharmacology
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Osteoblasts / cytology
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Osteoblasts / drug effects*
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Osteocalcin / genetics
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RNA, Messenger
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Tumor Necrosis Factor-alpha / pharmacology
Substances
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Hydrazines
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Interleukin-1
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Nitrogen Oxides
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RNA, Messenger
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Tumor Necrosis Factor-alpha
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Osteocalcin
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Nitroprusside
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Nitric Oxide
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1,1-diethyl-2-hydroxy-2-nitrosohydrazine
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Nitric Oxide Synthase
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Alkaline Phosphatase
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Cyclic GMP
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Dinoprostone