Direct action of nitric oxide on osteoblastic differentiation

FEBS Lett. 1997 Jun 30;410(2-3):238-42. doi: 10.1016/s0014-5793(97)00597-8.

Abstract

The effect of nitric oxide (NO) on osteoblastic differentiation was examined in cultured mouse osteoblasts. Interleukin-1beta and tumor necrosis factor-alpha expressed inducible NO synthase gene with little effect on constitutive NO synthase gene. These cytokines increased NO production, which was inhibited by L-NMMA pretreatment, and decreased alkaline phosphatase (AIPase) activity, which was not restored by L-NMMA. Furthermore, NO donors, sodium nitroprusside and NONOate dose-dependently elevated AIPase activity and expression of osteocalcin gene. These results suggest that NO directly facilitates osteoblastic differentiation and the cytokine-induced inhibition of AIPase activity is mediated via mechanism other than NO.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / biosynthesis
  • Animals
  • Cell Differentiation / drug effects
  • Cells, Cultured
  • Cyclic GMP / biosynthesis
  • Dinoprostone / biosynthesis
  • Hydrazines / pharmacology
  • Interleukin-1 / pharmacology
  • Mice
  • Nitric Oxide / pharmacology*
  • Nitric Oxide Synthase / genetics
  • Nitrogen Oxides
  • Nitroprusside / pharmacology
  • Osteoblasts / cytology
  • Osteoblasts / drug effects*
  • Osteocalcin / genetics
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha / pharmacology

Substances

  • Hydrazines
  • Interleukin-1
  • Nitrogen Oxides
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha
  • Osteocalcin
  • Nitroprusside
  • Nitric Oxide
  • 1,1-diethyl-2-hydroxy-2-nitrosohydrazine
  • Nitric Oxide Synthase
  • Alkaline Phosphatase
  • Cyclic GMP
  • Dinoprostone