Adaptation of the primer extension preamplification (PEP) reaction for preimplantation diagnosis: single blastomere analysis using short PEP protocols

K Sermon; W Lissens; H Joris; A Van Steirteghem; I Liebaers

doi:10.1093/molehr/2.3.209

Adaptation of the primer extension preamplification (PEP) reaction for preimplantation diagnosis: single blastomere analysis using short PEP protocols

Mol Hum Reprod. 1996 Mar;2(3):209-12. doi: 10.1093/molehr/2.3.209.

Authors

K Sermon¹, W Lissens, H Joris, A Van Steirteghem, I Liebaers

Affiliation

¹ Centre for Medical Genetics, Dutch-speaking Brussels Free University, Belgium.

PMID: 9238681
DOI: 10.1093/molehr/2.3.209

Abstract

Primer extension preamplification (PEP) was first described as a method for whole genome amplification, starting from a single cell, originally a spermatozoon, in order to perform genetic recombination studies. Its usefulness for preimplantation diagnosis was shown soon after; the only drawback was the length of the procedure (> 14 h). We have developed a shorter PEP protocol for single human blastomeres, enabling us to examine several genetic loci of interest in human genetic diseases with a good amplification efficiency.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Blastomeres / physiology*
Cystic Fibrosis / genetics
DNA Primers*
Embryonic Development*
Female
Fertilization in Vitro / methods
Humans
Polymerase Chain Reaction / methods*
Pregnancy
Tay-Sachs Disease / genetics

Substances

DNA Primers