Constitutive expression of calmodulin-binding phosphoprotein GAP-43 in rat serotonergic and noradrenergic cell groups which project to the spinal cord

Neurochem Res. 1997 Aug;22(8):985-93. doi: 10.1023/a:1022474826040.


In situ hybridization was combined with serotonin (5-hydroxytryptamine, 5-HT) or tyrosine hydroxylase immunocytochemistry and with Fluoro-Gold retrograde labeling of bulbo-spinal pathways in order to investigate the expression of GAP-43 mRNA in monoamine cell groups of the adult rat brain stem. Consistent with previous reports, GAP-43 mRNA was observed in serotonin and dopamine cell groups in the pons. In addition, GAP-43 expressing cells were observed in all the major monoamine cell groups in the medulla. Thus the B1, B2 and B3 serotonin cell groups all showed high GAP-43 expression in all contained many GAP-43 expressing serotonin cells with spinal cord projections. The A1, A2, A5 and A6 noradrenaline cell groups also showed high GAP-43 expression, although cells with spinal cord projections were largely restricted to the A5 group and A6 subcoeruleus region. In all areas, GAP-43 expressing cells with spinal cord projections were also observed which were not serotonergic or noradrenergic.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain Stem / metabolism*
  • Calmodulin-Binding Proteins / biosynthesis*
  • Calmodulin-Binding Proteins / metabolism
  • Dopamine / metabolism
  • Fluorescent Antibody Technique
  • GAP-43 Protein
  • In Situ Hybridization, Fluorescence
  • Male
  • Medulla Oblongata / cytology
  • Medulla Oblongata / enzymology
  • Medulla Oblongata / metabolism
  • Membrane Glycoproteins / biosynthesis*
  • Membrane Glycoproteins / metabolism
  • Nerve Tissue Proteins / biosynthesis*
  • Nerve Tissue Proteins / metabolism
  • Norepinephrine / physiology*
  • Oligonucleotide Probes
  • Pons / cytology
  • Pons / enzymology
  • Pons / metabolism
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Wistar
  • Serotonin / metabolism
  • Serotonin / physiology*
  • Spinal Cord / metabolism*
  • Tyrosine 3-Monooxygenase / metabolism


  • Calmodulin-Binding Proteins
  • GAP-43 Protein
  • Membrane Glycoproteins
  • Nerve Tissue Proteins
  • Oligonucleotide Probes
  • RNA, Messenger
  • Serotonin
  • Tyrosine 3-Monooxygenase
  • Dopamine
  • Norepinephrine