Periodontal repair in dogs: evaluation of rhBMP-2 carriers

Int J Periodontics Restorative Dent. 1996 Dec;16(6):524-37.

Abstract

This study evaluated candidate carriers for recombinant human bone morphogenetic protein-2 (rhBMP-2)-driven periodontal regeneration. Previous experiments indicated the ability of rhBMP-2 in a particulate delivery system to result in substantial regeneration of bone and periodontal regeneration. In the present study, canine demineralized bone matrix (DBM), bovine deorganified crystalline bone matrix (Bio-Oss), an absorbable collagen sponge (ACS) of type I bovine collagen, poly(D,L-lactide-co-glycolide) microparticles (PLGA), and polylactic acid granules (Drilac) were tested for their ability to support rhBMP-2 (0.2 mg/mL implant volume)-driven periodontal regeneration. The implants were tested in routine critical size canine supra-alveolar periodontal defects with transgingival tooth positioning. Contralateral defects in six beagle dogs were semirandomly assigned to receive: DBM/rhBMP-2, DBM (no rhBMP-2), Bio-Oss/rhBMP-2, ACS/rhBMP-2, PLGA/rhBMP-2, or Drilac/rhBMP-2. Animals were sacrificed 8 weeks postsurgery, and block sections of the defects were processed for light microscopy. Substantial bone regeneration was observed in all defects implanted with rhBMP-2. Other measures of periodontal healing, including cementum regeneration and presence of ankylosis, were more variable between the implants. DBM and Bio-Oss performed well as carriers for rhBMP-2-driven periodontal regeneration, although other impediments to their clinical use exist. This study indicates that qualities of the carrier system, including its space-maintaining capacity can affect the ability of rhBMP-2 to regenerate both alveolar bone and periodontal attachment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alveolar Bone Loss / surgery
  • Alveolar Bone Loss / therapy*
  • Animals
  • Biocompatible Materials
  • Bone Matrix
  • Bone Morphogenetic Proteins / administration & dosage*
  • Bone Morphogenetic Proteins / therapeutic use*
  • Bone Regeneration / drug effects*
  • Cattle
  • Collagen
  • Dogs
  • Drug Carriers
  • Evaluation Studies as Topic
  • Humans
  • Lactic Acid
  • Male
  • Minerals
  • Polyesters
  • Polyglycolic Acid*
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polymers
  • Prostheses and Implants
  • Recombinant Proteins / therapeutic use*
  • Root Resorption
  • Surgical Flaps*
  • Tooth Root / anatomy & histology

Substances

  • Bio-Oss
  • Biocompatible Materials
  • Bone Morphogenetic Proteins
  • Drug Carriers
  • Minerals
  • Polyesters
  • Polymers
  • Recombinant Proteins
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polyglycolic Acid
  • Lactic Acid
  • poly(lactide)
  • Collagen