A new prognostic strategy for gastric carcinoma: mRNA expression of tumor growth-related factors in endoscopic biopsy specimens

Ann Surg. 1997 Jul;226(1):35-42. doi: 10.1097/00000658-199707000-00005.


Objective: The study analyzed the prognostic value of the transcription of several tumor growth-related genes in gastric carcinoma biopsy specimens.

Summary background data: The nodal status is one of the most significant prognostic factors in gastric carcinoma. There are, however, no satisfactory parameters for the preoperative assessment of nodal status.

Methods: A reverse transcriptase-polymerase chain reaction analysis was used to analyze the transcription of several tumor growth-related genes in endoscopic biopsy specimens from 78 gastric carcinomas. The factors examined were cyclin D1, cyclin E, urokinase-type plasminogen activator, 72-kd type IV collagenase, vascular endothelial growth factor, platelet-derived growth factor-A (PDGF-A), transforming growth factor-beta, and interleukin-10. The relation between the mRNA expression and the clinical pathologic parameters was analyzed statistically.

Results: The incidence of PDGF-A (p = 0.010) and transforming growth factor-beta (p = 0.009) mRNA expression increased as the pathologic stage advanced. Nodal metastasis correlated with cyclin D1 (p = 0.045), cyclin E (p = 0.037), urokinase-type plasminogen activator (p = 0.047), and PDGF-A (p = 0.003) mRNA. Interestingly, the expression of PDGF-A mRNA showed a positive correlation (p = 0.004) with the early presence of lymph node metastases.

Conclusions: Tumor growth-related factor mRNA in biopsy specimens may be a new prognostic tool.

MeSH terms

  • Biopsy
  • Blotting, Southern
  • Carcinoma / metabolism*
  • Carcinoma / pathology
  • DNA Primers
  • Gastric Mucosa / pathology
  • Gene Expression
  • Growth Substances / biosynthesis*
  • Growth Substances / genetics
  • Humans
  • Neoplasm Staging
  • Polymerase Chain Reaction
  • Prognosis
  • RNA, Messenger / genetics
  • Stomach / pathology
  • Stomach Neoplasms / metabolism*
  • Stomach Neoplasms / pathology


  • DNA Primers
  • Growth Substances
  • RNA, Messenger