Expression of both Th1 and Th2 cytokines correlates with the histological rejection of MHC-matched and MHC-mismatched foetal pancreas allografts in mice

Immunol Cell Biol. 1997 Jun;75(3):303-9. doi: 10.1038/icb.1997.46.

Abstract

Foetal mouse pancreatic islet grafts were used to investigate differences in the histological appearance and cytokine expression pattern during acute rejection of fully MHC-mismatched, and MHC-matched but minor histocompatibility-mismatched (mH) allografts. Grafts of foetal islet tissue from non-obese diabetic mice under the kidney capsule of non-immunosuppressed MHC and mH-disparate BALB/c mice were rejected by day 9, whereas the rejection of only mH-disparate C3H tissue into CBA mice occurred between 11 and 15 days. In both situations enhanced expression of transcripts for Th1 (IL-2, IFN-gamma, TNF-beta) and Th2 cytokines (IL-4, IL-6, IL-10) was demonstrable at the peak of infiltration of immune cells into the graft site, indicating a close association of these cytokines with graft destruction. Besides these kinetic differences no variation in the expression pattern of the tested cytokines could be demonstrated, indicating that the allograft response in either combination leads to enhanced expression of pro-inflammatory cytokines which could contribute to graft destruction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cytokines / genetics*
  • DNA Primers / genetics
  • Fetal Tissue Transplantation / adverse effects
  • Fetal Tissue Transplantation / immunology*
  • Fetal Tissue Transplantation / pathology
  • Gene Expression
  • Graft Rejection / etiology*
  • Graft Rejection / immunology
  • Graft Rejection / pathology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred NOD
  • Pancreas Transplantation / adverse effects
  • Pancreas Transplantation / immunology*
  • Pancreas Transplantation / pathology
  • Polymerase Chain Reaction
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Th1 Cells / immunology
  • Th2 Cells / immunology
  • Time Factors
  • Transplantation, Homologous

Substances

  • Cytokines
  • DNA Primers
  • RNA, Messenger