Beta APP gene transfer into cultured human muscle induces inclusion-body myositis aspects

Neuroreport. 1997 Jul 7;8(9-10):2155-8. doi: 10.1097/00001756-199707070-00012.


Direct transfer of the beta-amyloid precursor protein (beta APP) gene into cultured normal human muscle, using recombinant adenovirus vector, was sufficient to induce several of the typical light microscopic, electron microscopic (EM), and EM-immunochemical aspects of the inclusion-body myositis (IBM) phenotype, including congophilia, clusters of amyloid-beta-positive 6-10 nm filaments, and 15-21 nm tubulofilamentous inclusions in the nuclei. Our results suggest that excessive production of intracellular beta APP may play an important role in the pathogenic cascade leading to the IBM phenotype.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amyloid beta-Protein Precursor / genetics*
  • Cells, Cultured
  • Gene Transfer Techniques*
  • Humans
  • Microscopy, Electron
  • Muscle Fibers, Skeletal / drug effects
  • Muscle Fibers, Skeletal / ultrastructure*
  • Myositis / chemically induced*


  • Amyloid beta-Protein Precursor