Gluten-sensitive enteropathy in Irish setter dogs: characterisation of jejunal microvillar membrane proteins by two-dimensional electrophoresis

Res Vet Sci. Mar-Apr 1997;62(2):191-3. doi: 10.1016/s0034-5288(97)90146-6.

Abstract

This study investigated whether gluten-sensitive enteropathy (GSE) in Irish setter dogs was associated with underlying structural abnormalities of microvillar membrane proteins. Jejunal biopsies taken from eight-month-old GSE-affected dogs reared on a normal, gluten-containing diet exhibited partial villous atrophy and contained more intra-epithelial lymphocytes than controls. The morphological abnormalities were reversed by feeding a gluten-free diet for five months and the changes were accompanied by an increase in the mucosal activity of the microvillar hydrolases, particularly aminopeptidase N and dipeptidyl aminopeptidase IV, which reverted to pre-treatment levels after a gluten challenge. Two-dimensional electrophoresis of microvillar membrane proteins isolated from GSE-affected dogs revealed an essentially normal protein map that was comparable to controls. The exception was an intense 85 kDa protein spot that diminished when the affected dogs were fed a gluten-free diet and re-intensified after a gluten challenge.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / analysis
  • Aminopeptidases / analysis
  • Animals
  • Atrophy / pathology
  • Atrophy / veterinary
  • Biopsy / veterinary
  • Celiac Disease / metabolism
  • Celiac Disease / pathology
  • Celiac Disease / veterinary*
  • Dog Diseases / genetics
  • Dog Diseases / metabolism*
  • Dog Diseases / pathology*
  • Dogs
  • Electrophoresis, Gel, Two-Dimensional / methods
  • Electrophoresis, Gel, Two-Dimensional / veterinary
  • Glutens / pharmacology
  • Hydrolases / analysis
  • Jejunum / chemistry*
  • Jejunum / pathology
  • Jejunum / ultrastructure*
  • Lymphocytes / pathology
  • Membrane Proteins / analysis*
  • Membrane Proteins / metabolism
  • Microvilli / chemistry
  • Microvilli / metabolism
  • Microvilli / ultrastructure

Substances

  • Membrane Proteins
  • Glutens
  • Hydrolases
  • Alkaline Phosphatase
  • Aminopeptidases