Bidirectional transport by distinct populations of COPI-coated vesicles

Cell. 1997 Jul 25;90(2):335-49. doi: 10.1016/s0092-8674(00)80341-4.


Electron microscope immunocytochemistry reveals that both anterograde-directed (proinsulin and VSV G protein) and retrograde-directed (the KDEL receptor) cargo are present in COPI-coated vesicles budding from every level of the Golgi stack in whole cells; however, they comprise two distinct populations that together can account for at least 80% of the vesicles budding from Golgi cisternae. Segregation of anterograde- from retrograde-directed cargo into distinct sets of COPI-coated vesicles is faithfully reproduced in the cell-free Golgi transport system, in which VSV G protein and KDEL receptor are packaged into separable vesicles, even when budding is driven by highly purified coatomer and a recombinant ARF protein.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Animals, Newborn
  • Biological Transport / physiology
  • Cell-Free System
  • Cells, Cultured
  • Coated Vesicles / chemistry*
  • Coated Vesicles / metabolism*
  • Coated Vesicles / ultrastructure
  • Coatomer Protein
  • Golgi Apparatus / chemistry
  • Golgi Apparatus / metabolism
  • Golgi Apparatus / ultrastructure
  • Membrane Proteins / analysis
  • Membrane Proteins / metabolism*
  • Microscopy, Immunoelectron
  • Pancreas / cytology
  • Rats
  • Receptors, Peptide / analysis


  • Coatomer Protein
  • KDEL receptor
  • Membrane Proteins
  • Receptors, Peptide