Cloning and Sequence Analysis of the Fimbriae Associated Protein (Fap) Gene From Actinobacillus Actinomycetemcomitans

Microb Pathog. 1997 Aug;23(2):63-9. doi: 10.1006/mpat.1997.0137.

Abstract

Fimbrial-associated protein, an attachment factor of Actinobacillus actinomycetemcomitans, was genetically analysed by cloning. The plasmid obtained was found to harbor a 1.7kb fragment which contained a 228bp open reading frame encoding 76 amino acids (7.970kDa). The fimbriae associated protein gene (fap) was strongly expressed in fimbriated A. actinomycetemcomitans 310a but not in non-fimbriated strains.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aggregatibacter actinomycetemcomitans / genetics*
  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • DNA, Bacterial
  • Fimbriae Proteins*
  • Fimbriae, Bacterial*
  • Gene Expression
  • Genes, Bacterial
  • Molecular Sequence Data
  • Sequence Analysis, DNA

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • fimbriae-associated protein, Actinobacillus
  • Fimbriae Proteins

Associated data

  • GENBANK/D83053