Mouse macrophage development in the absence of the common gamma chain: defining receptor complexes responsible for IL-4 and IL-13 signaling

Eur J Immunol. 1997 Jul;27(7):1762-8. doi: 10.1002/eji.1830270725.


The common gamma chain (gamma c) forms a critical component of the receptors for interleukins (IL)-2, IL-4, IL-7, IL-9, and IL-15. We analyzed gamma c-deficient mice to define a role for gamma c signaling in the development and function of the macrophage lineage. No major differences in absolute cell numbers, cell surface phenotype, or in vitro function of gamma c- compared to gamma c+ macrophages were observed. We therefore conclude that signaling through the gamma c chain is not essential for the differentiation of mouse macrophages. Although B and T cells require gamma c for IL-4 responses, IL-4 up-regulated major histocompatibility class II molecules and inhibited nitric oxide production from gamma c- macrophages following stimulation with lipopolysaccharide and interferon-gamma. gamma c- macrophages could also respond to IL-13, consistent with the model of a type II IL-4 receptor alpha/IL-13R which can function in the absence of gamma c. Both IL-4 and IL-13 responses could be completely inhibited with the mouse IL-4 antagonist OY, suggesting that all of the observed IL-13 responses pass through the type II receptor, making it the primary signaling receptor complex for IL-13 in mouse macrophages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation / genetics
  • Cell Differentiation / immunology
  • Female
  • Immunophenotyping
  • Interleukin-13 / antagonists & inhibitors
  • Interleukin-13 / metabolism*
  • Interleukin-13 / physiology
  • Interleukin-4 / antagonists & inhibitors
  • Interleukin-4 / metabolism*
  • Interleukin-4 / physiology
  • Macrophages, Peritoneal / cytology*
  • Macrophages, Peritoneal / drug effects
  • Macrophages, Peritoneal / metabolism*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Mutant Strains
  • Nitric Oxide / antagonists & inhibitors
  • Nitric Oxide / biosynthesis
  • Receptors, Interleukin / deficiency*
  • Receptors, Interleukin / genetics*
  • Receptors, Interleukin / physiology
  • Signal Transduction / genetics
  • Signal Transduction / immunology*


  • Interleukin-13
  • Receptors, Interleukin
  • Interleukin-4
  • Nitric Oxide