Prolonged survival of mouse skin allografts in recipients treated with donor splenocytes and antibody to CD40 ligand

Transplantation. 1997 Jul 27;64(2):329-35. doi: 10.1097/00007890-199707270-00026.

Abstract

Combined treatment with antibody against CD40 ligand and one transfusion of donor splenocytes prolonged survival of fully mismatched BALB/c skin allografts on C57BL/6 recipients, with approximately 20% of grafts surviving > 100 days. In vitro alloresponsiveness in treated animals was reduced in the immediate post-transplantation period, but by day 100 was increased despite the presence of a successful allograft. The presence of alloreactivity on day 100 was confirmed in vivo by adoptive transfer, which suggests that our protocol had induced either a state of "split tolerance" or "graft accommodation." Mice with skin grafts that had survived for > or = 100 days revealed no evidence of lymphoid chimerism. Treatment with donor splenocytes and antibody against CD40 ligand permits long-term survival of highly antigenic donor skin allografts despite the presence of functionally intact alloreactive lymphocytes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adoptive Transfer
  • Animals
  • Antibodies, Monoclonal / therapeutic use*
  • CD40 Ligand
  • Chimera
  • DNA / analysis
  • Graft Rejection / prevention & control
  • Graft Survival / immunology
  • Hematopoietic Stem Cells / physiology
  • Immune Tolerance / immunology
  • Lymph Nodes / chemistry
  • Male
  • Membrane Glycoproteins / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Polymerase Chain Reaction
  • Skin Transplantation / immunology*
  • Spleen / chemistry
  • Spleen / cytology*

Substances

  • Antibodies, Monoclonal
  • Membrane Glycoproteins
  • CD40 Ligand
  • DNA