Altered expression of NPY-Y1 receptors in kainic acid induced epilepsy in rats

Neurosci Lett. 1997 Jul 18;230(2):129-32. doi: 10.1016/s0304-3940(97)00492-8.

Abstract

Kainic acid-induced limbic seizures cause lasting increases in neuropeptide Y (NPY) expression in hippocampal granule cells/mossy fibers. The expression of NPY-Y1 receptors in these neurons were investigated, using in situ hybridization for Y1 mRNA and receptor autoradiography with the Y1-specific ligand [125I][Pro34]PYY. Six hours after kainic acid-induced seizures, Y1 receptor mRNA levels decreased by 80% in granule cells and concomitantly increased (by 75%) in CA2 pyramidal neurons. Subsequently, persistent decreases in Y1 mRNA were seen, both in the stratum granulosum and in CA2. Changes in mRNA concentrations were accompanied by a transient, although non-significant, increase in [125I][Pro34]PYY binding in the molecular layer of the dentate gyrus after 4-6 h which was succeeded by a lasting decrease in binding which indicates a persistent down-regulation of Y1 receptors in hippocampal areas in kainic acid-induced epilepsy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autoradiography
  • Dentate Gyrus / metabolism
  • Epilepsy / chemically induced
  • Epilepsy / metabolism*
  • Hippocampus / metabolism*
  • Iodine Radioisotopes
  • Kainic Acid
  • Male
  • Neurons / metabolism*
  • Peptide YY
  • Peptides / metabolism
  • Pyramidal Cells / metabolism*
  • RNA, Messenger / biosynthesis
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Neuropeptide Y / biosynthesis*
  • Time Factors
  • Transcription, Genetic*

Substances

  • Iodine Radioisotopes
  • Peptides
  • RNA, Messenger
  • Receptors, Neuropeptide Y
  • neuropeptide Y-Y1 receptor
  • Peptide YY
  • Kainic Acid