Comparison of cytogenetics, cytokine secretion, and oncogene expression in primary cultures of renal carcinoma cells

Oncology. Sep-Oct 1997;54(5):429-37. doi: 10.1159/000227730.

Abstract

We compared the cytogenetic pattern of 20 different primary tumor cell cultures (PTCC) of renal cell carcinoma (RCC) to their cytokine secretion and oncogene expression. High secretion of IL-6 (gene locus on chromosome 7p21-p14) was correlated with the gain of an additional chromosome 7. Structural changes involving chromosome 5q22, the site of the GM-CSF gene, were matched with the high secretion of GM-CSF in PTCC. No such association was found for beta 2-microglobulin, TGF-beta 1, TNF-alpha, IL-8, and oncogenes, such as c-fos, c-myc, and pan-ras. Our approach may be useful in simultaneously analyzing several factors contributing to tumor progression and may contribute to understanding the multistep development of RCC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Carcinoma, Renal Cell / genetics
  • Carcinoma, Renal Cell / metabolism*
  • Cytokines / biosynthesis*
  • Cytokines / genetics
  • Drug Resistance, Multiple
  • Drug Resistance, Neoplasm
  • Epidermal Growth Factor / biosynthesis
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Granulocyte-Macrophage Colony-Stimulating Factor / biosynthesis
  • HSP70 Heat-Shock Proteins / biosynthesis
  • Humans
  • Intercellular Adhesion Molecule-1 / biosynthesis
  • Interleukins / biosynthesis
  • Male
  • Middle Aged
  • Proto-Oncogenes* / genetics
  • Transforming Growth Factor alpha / biosynthesis
  • Tumor Cells, Cultured
  • Tumor Necrosis Factor-alpha / biosynthesis
  • Tumor Suppressor Protein p53 / biosynthesis

Substances

  • Cytokines
  • HSP70 Heat-Shock Proteins
  • Interleukins
  • Transforming Growth Factor alpha
  • Tumor Necrosis Factor-alpha
  • Tumor Suppressor Protein p53
  • Intercellular Adhesion Molecule-1
  • Epidermal Growth Factor
  • Granulocyte-Macrophage Colony-Stimulating Factor